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| Registros recuperados: 23 | |
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| Colmenares,Cecilia; Méndez,Liber; Díaz-Bello,Zoraida; Alarcón de Noya,Belkisyolé. |
| La baja sensibilidad de la coprología en el diagnóstico de la fasciolosis humana ha motivado el desarrollo del inmunodiagnóstico. El antígeno de excreción-secreción de adultos de Fasciola hepatica (AFhES) en ELISA es adecuado para el screening, aunque sobrestima la prevalencia; cuando se utiliza en Western blot (WB) no muestra un buen reconocimiento de sus componentes. Para lograr una mejor preparación, se ultrafiltró el antígeno a través de membranas YM de 10, 30 y 50 kDa. Los retenidos (R) se usaron en ELISA y WB. La mayor discriminación entre positivos y negativos en ELISA y la mejor resolución en el reconocimiento al antígeno en WB, se logró con la fracción R50. Se destacan las moléculas de 9, 14, 65 kDa y la región alrededor de 27 kDa, detectadas con... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fasciolosis; Antígeno; Ultrafiltración; Inmunodiagnóstico; ELISA; Western blot. |
| Ano: 2007 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572007000200010 |
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| Bruder,Juliana; Godry,Renata Cristina; Takei,Kioko; Cunha,Regina Ayr Florio da. |
| Mycoplasmas are a heterogeneous group of the smallest organisms capable of self replication and are known to cause many detrimental diseases in both animals and humans. These wall-less prokaryotes are enveloped by a lipoprotein membrane and their small genomes are sufficient to synthesize molecules required for growth and self-replication. Among sixteen species isolated from humans, Mycoplasma pneumoniae, an agent of primary atypical pneumonia, and the urogenital tract species Mycoplasma hominis,Ureaplasma urealyticum and Ureaplasma parvum have been confirmed to be pathogenic. Mycoplasma penetrans and Mycoplasma fermentans, which are species associated with HIV, have been investigated mainly in research laboratories. In this study we have characterized... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoplasma penetrans; Mycoplasma fermentans; LAMP-proteins; ELISA; Western blot. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200007 |
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| Martínez-Oliva,Brenda Gisela; Montaño-Valenzuela,Karen Jimena; Rodriguez-Herbas,Patricia; Flores-León,Amilcar Alejandr; Grados-Torrez,Ricardo Enrique. |
| En Bolivia, el algoritmo diagnóstico convencional para VIH utiliza como prueba confirmatoria el Western blot; debido a dificultades con el costo, tiempo y centralización del diagnóstico confirmatorio, el Programa Nacional ITS/VIH/SIDA-HV propuso un algoritmo nuevo que utiliza solo dos pruebas rápidas y ELISA Ag/Ac. Sesenta muestras de suero fueron procesadas con ambos algoritmos y se determinó el resultado verdadero de las muestras de acuerdo con ambos algoritmos y la carga viral. La sensibilidad, especificidad, valores predictivos, índice Kappa y chi-cuadrado de Pearson fueron calculados. El algoritmo convencional reportó: sensibilidad 96,3%, especificidad 100%, valor predictivo positivo 100% y valor predictivo negativo 97% el algoritmo nuevo presentó... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diagnóstico de VIH; Prueba rápida; Western blot; Carga viral. |
| Ano: 2018 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572018000200007 |
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| Elkady,Ayman I.. |
| There is an urgent need to improve the clinical management of non-small cell lung cancer (NSCLC), one of the most frequent causes of cancer-related deaths in men and women worldwide. Rhazya stricta, an important medicinal plant used in traditional Oriental medicine, possesses anti-oxidant, anti-carcinogenic and free radical scavenging properties. This study was done to explore the potential anticancer activity of a crude alkaloid extract of R. stricta (CAERS) against the NSCLC line A549. CAERS markedly suppressed the growth of A549 cells and considerably enhanced the anti-proliferative potential of cisplatin. CAERS-mediated inhibition of A549 cell growth correlated with the induction of apoptosis that was accompanied by numerous morphological changes, DNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoptosis; Lung cancer; Medicinal plant; RT-PCR; Western blot. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100003 |
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| NICKEL,OSMAR; FAJARDO,THOR V. M.; ARAGÃO,FRANCISCO J. L.; CHAGAS,CÉSAR M.; KUHN,GILMAR B.. |
| An isolate of Grapevine virus B (GVB), obtained by indexing Vitis labrusca and V. vinifera grapevines on the indicator LN33, was transmitted mechanically to several Nicotiana species. The virus was partially purified from N. cavicola and the coat protein estimated at 23 kDa by SDS-PAGE. In negatively stained leaf extracts of experimentally inoculated N. cavicola and N. occidentalis, flexuous particles with cross banding were observed, predominantly measuring 750-770 x 12 nm, with a modal length of 760 nm. Decoration indicated a clear, positive reaction against AS-GVB. In DAS-ELISA, GVB was detected in N. cavicola and grapevine extracts, and Western blots showed homologous and cross reaction of GVB and GVA antisera with GVB coat protein. Using specific... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GVB; Vitis spp.; Vitivirus; Electron microscopy; ELISA; Western blot; GVA. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000300007 |
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| Ramos,Márcio Viana; Bomfim,Liezelotte Rezende; Bandeira,; Debray,Henri. |
| Cratylia floribunda seeds were ground and the clean crude saline extract was fractionated into albumin, globulin, prolamin, acidic and basic glutelin protein fractions. These protein fractions were examined for the presence of an endogenous lectin receptor by SDS-polyacrylamide gel electrophoresis, western blot, affinity chromatography on a Sepharose 4B-Cratylia floribunda (CFL) lectin column and kinetic analysis in real time by surface plasmon resonance (SPR). Prolamin was the richest protein fraction although very poor in haemagglutinating activity. Basic glutelin was far the less interesting fraction for lectin activity and protein content, even though this fraction contains considerable amounts of carbohydrates. Lectin was present in all protein... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Protein-carbohydrate interaction; Surface plasmon resonance; Western blot. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000300003 |
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| Vrat Kamboj,Dev; Nema,Vijay; Kumar Pandey,Arun; Kumar Goel,Ajay; Singh,Lokendra. |
| Staphylococcal food poisoning (SFP) is caused by the members of superantigen family called staphylococcal enterotoxins (SEs). About 20 different types of SEs are produced by Staphylococcus aureus out of which type A (SEA), B (SEB), C (SEC) and D (SED) are commonly implicated in SFP. Among these, SEB is the most potent toxin and has also gained the status of biological warfare (BW) agent. Therefore, detection of SEB is of utmost importance. Any immunological detection system for SEB requires specific and sensitive antibodies which inturn depends on the purity of the SEB. In the present investigation, seb gene of S. aureus was cloned and expressed in E. coli along with biotin as fusion partner to facilitate the purification process. The yield of purified... |
| Tipo: Journal article |
Palavras-chave: Affinity purification; Biotin fusion; ELISA; Gene cloning; SEB; Western blot. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500010 |
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| Beck,S.T.; Leite,O.M.; Arruda,R.S.; Ferreira,A.W.. |
| Much effort has been devoted to the identification of immunologically important antigens of Mycobacterium tuberculosis and to the combination of target antigens to which antibodies from serum of tuberculous patients could react specifically. We searched for IgG antibodies specific for antigens of 45 to 6 kDa obtained after sonication of the well-characterized wild M. tuberculosis strain in order to detect differences in the antibody response to low molecular weight antigens from M. tuberculosis between patients with pulmonary tuberculosis and contacts. Specific IgG antibodies for these antigens were detected by Western blot analysis of 153 serum samples collected from 51 patients with confirmed pulmonary tuberculosis. Three samples were collected from each... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Western blot; Profile; Tuberculosis; Antigens. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400013 |
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| Picot, Sandy; Faury, Nicole; Arzul, Isabelle; Chollet, Bruno; Renault, Tristan; Morga, Benjamin. |
| The Pacific oyster, Crassostrea gigas, is a mollusk bivalve commercially important as a food source. Pacific oysters are subjected to stress and diseases during culture. The autophagy pathway is involved in numerous cellular processes, including responses to starvation, cell death, and microorganism elimination. Autophagy also exists in C. gigas, and plays a role in the immune response against infections. Although this process is well-documented and conserved in most animals, it is still poorly understood in mollusks. To date, no study has provided a complete overview of the molecular mechanism of autophagy in mollusk bivalves. In this study, human and yeast ATG protein sequences and public databases (Uniprot and NCBI) were used to identify protein members... |
| Tipo: Text |
Palavras-chave: Autophagy; Autophagy related; Crassostrea gigas; Data mining; Immunohistochemistry; Real-time PCR; Western blot. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00606/71785/70270.pdf |
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| Zuccari,D.A.P.C; Castro,R; Jardim,B.V; Mancini,U.M; Polachini,G.M. |
| Forty-eight cases of canine mammary cancer were investigated to evaluate the immunohistochemical distribution of the γ2 chain of laminin-332. Tumor cells were compared to a pool of normal mammary tissues using quantitative RT-PCR. The western blot was performed in eight tumor samples as complementary test to evaluate protein integrity. Immunohistochemistry experiments showed negative, focal, and weak expression of laminin-332 γ2 in tumors with the worst prognosis. Quantitative PCR revealed downregulation of the gene in 27 (56.2%) of the animals. Out of the 16 dogs with γ2 chain overexpression, seven were still alive. The western blot results showed bands generation of 36, 50, and 98kDa, suggesting degradation of laminin-332 γ2 in malignant tumors. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Adhesion molecule; Cancer; Western blot; RT-PCR. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000100005 |
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| França,S.A.; Mol,J.P.S.; Costa,E.A.; Silva,A.P.C.; Xavier,M.N.; Tsolis,R.M.; Reis,J.K.P.; Paixão,T.A.; Santos,R.L.. |
| Brucella ovis is a major cause of epididymitis in sexually mature rams, resulting in subfertility, infertility, and economic losses for the sheep industry worldwide. The aim of this study was to develop an indirect ELISA (iELISA) using recombinant proteins, namely rBoP59 and rBP26, as antigens for serological diagnosis of B. ovisinfection. The BoP59 and BP26 recombinant proteins were expressed in E. coli and purified by affinity chromatography. Antigenicity was tested by Western blot and iELISA. Standardization of iELISA was performed with 500ng and 1µg BoP59 and rBP26 per well, testing serum from uninfected and experimentally infected rams. rBP26 was effective in distinguishing positive from negative rams. The rBP26 iELISA developed in this study is the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucella ovis; Brucellosis; ELISAi; BP26; Western blot. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601695 |
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| Wei,Jian; Liang,Bing-Sheng. |
| Activated inhibitor of nuclear factor-κB kinase β (IKKβ) is necessary and sufficient for denervated skeletal muscle atrophy. Although several studies have shown that Mg2+/Mn2+-dependent protein phosphatase 1B (PPM1B) inactivated IKKβ, few studies have investigated the role of PPM1B in denervated skeletal muscle. In this study, we aim to explore the expression and significance of PPM1B and phosphorylated IKKβ (P-IKKβ) during atrophy of the denervated gastrocnemius. Thirty young adult female Wistar rats were subjected to right sciatic nerve transection and were sacrificed at 0 (control), 2, 7, 14, and 28 days after denervation surgery. The gastrocnemius was removed from both the denervated and the contralateral limb. The muscle wet weight ratio was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Muscular atrophy; Denervation; PPM1B; P-IKKβ; IKKβ; RT-PCR; Western blot. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800004 |
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| Alves,L.A.O.; Teixeira,M.F.S.; Pinheiro,A.A.; Pinheiro,R.R.; Dias,R.P.; Brito,R.L.L.; Lopes Júnior,C.A.F.; Bezerra Júnior,R.Q.; Azevedo,D.A.A.. |
| Este estudo teve como objetivo produzir um antígeno (Ag) a partir de cultura de células de membrana sinovial caprina (MSC) infectadas com o vírus de artrite encefalite caprina (CAEV), pela técnica de microfiltração seriada, substituindo a ultracentrifugação em colchão de sacarose (UCCS) para utilização em ELISA indireto (ELISA-i). Amostras de 188 soros caprinos, que previamente foram testados pelo Western blot (WB) com Ag UCCS, foram submetidas à análise pelo ELISA-i com o novo antígeno produzido, que mostrou concordância de 92% em relação ao antígeno UCCS. A sensibilidade e a especificidade do ELISA em relação ao WB foram de 95,6% e 88,5%, respectivamente. A nova técnica, criada a partir de microfiltrações, mostrou-se efetiva e de baixo custo para o... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Caprino; CAEV; ELISA; Lentivírus de pequenos ruminantes; Western blot. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400021 |
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| Radaelli,Paula; Fajardo,Thor Vinícius Martins; Nickel,Osmar; Eiras,Marcelo; Pio-Ribeiro,Gilvan. |
| The objective of this work was to produce and characterize specific antisera against Brazilian isolates of Grapevine leafroll-associated virus 2 (GLRaV-2) and Grapevine virus B (GVB), developed from expressed coat proteins (CPs) in Escherichia coli, and to test their possible use for the detection of these two viruses in diseased grapevines. The coat protein (CP) genes were RT-PCR-amplified, cloned and sequenced. The CP genes were subsequently subcloned, and the recombinant plasmids were used to transform E. coli cells and express the coat proteins. The recombinant coat proteins were purified, and their identities were confirmed by SDS-PAGE and Western blot and used for rabbit immunizations. Antisera raised against these proteins were able to recognize the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vitis; GLRaV-2; GVB; Indirect ELISA; Recombinant protein; Western blot. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001000020 |
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| BULAT,TANJA; KETA,OTILIJA; KORIĆANAC,LELA; ŽAKULA,JELENA; PETROVIĆ,IVAN; RISTIĆ-FIRA,ALEKSANDRA; TODOROVIĆ,DANIJELA. |
| ABSTRACT Ionizing radiation induces DNA double strand breaks (DSBs) that trigger phosphorylation of the histone protein H2AX (γH2AX). Immunofluorescent staining visualizes formation of γH2AX foci, allowing their quantification. This method, as opposed to Western blot assay and Flow cytometry, provides more accurate analysis, by showing exact position and intensity of fluorescent signal in each single cell. In practice there are problems in quantification of γH2AX. This paper is based on two issues: the determination of which technique should be applied concerning the radiation dose, and how to analyze fluorescent microscopy images obtained by different microscopes. HTB140 melanoma cells were exposed to γ-rays, in the dose range from 1 to 16 Gy. Radiation... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ApoTome software; AxioImagerA1 microscope; Immunofluorescence microscopy; Western blot; ΓH2AX. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100127 |
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| Registros recuperados: 23 | |
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